Coutts R H, Rigden J E, Slabas A R, Lomonossoff G P, Wise P J
Department of Biology, Imperial College of Science, Technology and Medicine, London, U.K.
J Gen Virol. 1991 Jul;72 ( Pt 7):1521-9. doi: 10.1099/0022-1317-72-7-1521.
The complete sequence of the RNA genome of tobacco necrosis virus strain D (TNV-D) consisting of 3759 nucleotides has been determined. The positive strand contains five open reading frames (ORFs). The 5'-proximal ORF encodes a 22K protein terminating with an amber codon which may be read through to produce a 82K protein (p82). Two small centrally located ORFs each encode two out-of-frame 7K proteins (p7a and p7b). The 3'-proximal ORF encodes the 29K coat protein (CP), the N terminus of which has been sequenced directly. The genomic organization of TNV-D is very similar to that of TNV-A but differs in the placement of the p7a ORF, which does not overlap the p82 ORF in TNV-D, and in the absence of an ORF downstream of the CP gene in TNV-D. The p82 ORF shows extensive sequence similarity with the putative polymerases of the carmovirus group. This ORF is also as closely related to the corresponding ORF of TNV-A as it is to the corresponding ORF of the tombusvirus cucumber necrosis virus. The amino acid sequence of the TNV-D CP gene is similar to both the TNV-A and southern bean mosaic virus CP genes. Of the two p7 ORFs, p7a exhibits amino acid sequence similarity with corresponding proteins from TNV-A, melon necrotic spot virus, carnation mottle virus, turnip crinkle virus and maize chlorotic mottle virus, whereas the p7b ORF appears to be unique to TNV-A and TNV-D. Only the 3'-terminal three nucleotides of TNV-D genomic RNA are identical to the 3'-terminal nucleotides of the TNV satellite virus.
已确定烟草坏死病毒D株（TNV-D）由3759个核苷酸组成的RNA基因组的完整序列。正链包含五个开放阅读框（ORF）。5'近端ORF编码一个以琥珀密码子终止的22K蛋白，该密码子可能被通读以产生一个82K蛋白（p82）。两个位于中央的小ORF分别编码两个移码的7K蛋白（p7a和p7b）。3'近端ORF编码29K外壳蛋白（CP），其N端已直接测序。TNV-D的基因组结构与TNV-A非常相似，但在p7a ORF的位置上有所不同，在TNV-D中它不与p82 ORF重叠，并且在TNV-D的CP基因下游不存在ORF。p82 ORF与香石竹斑驳病毒组的推定聚合酶显示出广泛的序列相似性。该ORF与TNV-A的相应ORF以及番茄丛矮病毒黄瓜坏死病毒的相应ORF的关系同样密切。TNV-D CP基因的氨基酸序列与TNV-A和南方菜豆花叶病毒的CP基因相似。在两个p7 ORF中，p7a与来自TNV-A、甜瓜坏死斑点病毒、香石竹斑驳病毒、芜菁皱缩病毒和玉米褪绿斑驳病毒的相应蛋白表现出氨基酸序列相似性，而p7b ORF似乎是TNV-A和TNV-D所特有的。TNV-D基因组RNA仅3'末端的三个核苷酸与TNV卫星病毒的3'末端核苷酸相同。
