Namikawa K, Okazaki Y, Nishida S, Kimoto S, Akai F, Tomura T, Hashimoto S
Faculty of Pharmaceutical Sciences, Kinki University, Osaka, Japan.
Yakugaku Zasshi. 1993 Aug;113(8):591-5.
The amounts of myocardial metallothionein (MT) and heavy metal (Zn, Cu) levels during the early stage of the experimental myocardial infarction model induced by isoproterenol (Isp) administration were measured by an atomic absorption spectrophotometry. MT was measured by the Cd-hem method. Myocardial infarction was induced by the administration of 75 mg/kg i.p. of Isp to rats weighing 270 +/- 10 g. Thirty minutes after Isp injection, Zn and Cu levels began to decrease and 12 h later, reached the minimal values compared with the control value. The level of MT began to increase 3 h after the Isp injection and reached the maximal value at 12 h, although MT remained undetectable in the control myocardial tissue by the Cd-hem method. MT levels in the liver increased and total Zn and Cu were elevated compared with the control value 12 h after Isp administration. These results suggest that MT is produced in the myocardium after Isp administration, and that the roles of MT in the heart and the liver are different. It was thought that a rise in MT was induced for the protection of the myocardial cells to injury.
通过原子吸收分光光度法测定了异丙肾上腺素（Isp）诱导的实验性心肌梗死模型早期心肌金属硫蛋白（MT）含量和重金属（锌、铜）水平。MT采用镉-血红蛋白法测定。对体重270±10 g的大鼠腹腔注射75 mg/kg的Isp诱导心肌梗死。注射Isp 30分钟后，锌和铜水平开始下降，12小时后降至与对照值相比的最低值。Isp注射3小时后MT水平开始升高，12小时达到最大值，尽管通过镉-血红蛋白法在对照心肌组织中未检测到MT。Isp给药12小时后，肝脏中的MT水平升高，总锌和铜含量高于对照值。这些结果表明，Isp给药后心肌中产生了MT，并且MT在心脏和肝脏中的作用不同。认为MT升高是为了保护心肌细胞免受损伤。
