来自抗生链霉菌的磷脂酶D的结晶及初步X射线衍射研究。
Crystallization and preliminary X-ray diffraction studies of phospholipase D from Streptomyces antibioticus.
作者信息
Suzuki A, Kakuno K, Iwasaki Y, Yamane T, Yamane T
机构信息
Department of Biotechnology and Biomaterial Chemistry, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya 464-8603, Japan.
出版信息
Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):317-9. doi: 10.1107/S0907444998010592. Epub 1999 Jan 1.
Phospholipase D (E.C. 3.1.4.4) from Streptomyces antibioticus has been crystallized in six crystal forms using the hanging-drop vapour-diffusion method. The type III and V crystals belong to monoclinic and hexagonal systems, respectively. All of the other crystal forms, types I, II, IV and VI, belong to orthorhombic space group P212121. Of these four types, the type VI crystals are suitable for X-ray structure determination. Crystal data for type VI crystals are: a = 50.1, b = 98.7, c = 107.6 A, V = 532100 A3, Z = 4 and Vm = 2.47 A3 Da-1. Type VI crystals diffract to at least 2.3 A resolution. A total of 11295 independent reflections to 3 A resolution have been collected from a type VI crystal using a conventional X-ray source, and its structural analysis is currently being conducted using isomorphous replacement methods.
利用悬滴气相扩散法，已将来自抗生链霉菌的磷脂酶D（E.C. 3.1.4.4）结晶为六种晶体形式。III型和V型晶体分别属于单斜晶系和六方晶系。所有其他晶体形式，即I型、II型、IV型和VI型，均属于正交空间群P212121。在这四种类型中，VI型晶体适合进行X射线结构测定。VI型晶体的晶体数据为：a = 50.1，b = 98.7，c = 107.6 Å，V = 532100 ų，Z = 4，Vm = 2.47 ų Da⁻¹。VI型晶体的衍射分辨率至少为2.3 Å。使用传统X射线源从一个VI型晶体收集了总共11295个3 Å分辨率的独立反射，目前正在使用同晶置换法进行其结构分析。
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