Sheng H Z, Fields R D, Nelson P G
Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
J Neurosci Res. 1993 Aug 1;35(5):459-67. doi: 10.1002/jnr.490350502.
Electrical activity shapes development of the nervous system, presumably in part by regulating gene expression. A set of regulatory genes, immediate early genes (IEGs), which are responsive to a number of extrinsic cellular stimuli have been proposed to play a role in coupling such activity to gene expression. Using a semiquantitative polymerase chain reaction assay, we show that in dissociated mouse dorsal root ganglion neurons the expression of two IEGs, c-fos and nur/77, is differentially sensitive to patterns of electrical stimulation. Differences in c-fos activation did not correlate with the peak intracellular calcium [Ca++]i produced by the different stimulation patterns or with residual [Ca++]i following stimulation. However, the net increase in [Ca++]i (calcium time integral) was greater for the pulsed stimulus that activated c-fos (6 impulses/min), compared to the ineffective stimulus (12 impulses/2 min). This system of genes seems suited to mediating the coupling between electrical activity and other functional genes.
电活动塑造神经系统的发育，推测部分是通过调节基因表达来实现的。一组调节基因，即即刻早期基因（IEGs），对多种外在细胞刺激有反应，有人提出它们在将这种活动与基因表达相偶联中发挥作用。使用半定量聚合酶链反应分析，我们发现，在解离的小鼠背根神经节神经元中，两种即刻早期基因c-fos和nur/77的表达对电刺激模式有不同的敏感性。c-fos激活的差异与不同刺激模式产生的细胞内钙[Ca++]i峰值或刺激后的残余[Ca++]i均无关联。然而，与无效刺激（12次脉冲/2分钟）相比，激活c-fos的脉冲刺激（6次脉冲/分钟）使[Ca++]i的净增加（钙时间积分）更大。这组基因似乎适合介导电活动与其他功能基因之间的偶联。
