淀粉样蛋白、胶体和透明质酸中酸性粘多糖的组织化学研究。
Histochemical investigation of acid mucopolysaccharides in amyloid, colloid and hyaline.
作者信息
Sasai Y, Nagata M, Inokuchi K, Namba K
出版信息
Acta Histochem. 1978;62(2):223-36. doi: 10.1016/S0065-1281(78)80088-9.
The carbohydrate component in amyloid was histochemically compared with that in colloid or hyaline. Alcian blue, azure A, and periodic acid Schiff were used to stain mucopolysaccharides. In a further attempt to identify various polyanions, staining was carried out with alcian blue containing various concentrations of electrolytes. Methylation, saponification, acid hydrolysis, and digestion with streptomyces or testicular hyaluronidase, or chondroitinase ABC were also employed. The results obtained suggest that the presence of heparitin sulfate in amyloid, of chondroitin sulfate A in hyaline, and of chondroitin sulfate C in colloid.
对淀粉样蛋白中的碳水化合物成分与胶体或透明质中的碳水化合物成分进行了组织化学比较。使用阿尔辛蓝、天青A和过碘酸希夫试剂对粘多糖进行染色。为了进一步鉴定各种聚阴离子，用含有不同浓度电解质的阿尔辛蓝进行染色。还采用了甲基化、皂化、酸水解以及用链霉菌或睾丸透明质酸酶或软骨素酶ABC进行消化。所得结果表明，淀粉样蛋白中存在硫酸乙酰肝素，透明质中存在硫酸软骨素A，胶体中存在硫酸软骨素C。
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