人皮肤肥大细胞中透明质酸的组织化学显示

Histochemical demonstration of hyaluronic acid in human dermal mast cells.

作者信息

Sasai Y, Ishibashi M

出版信息

Tohoku J Exp Med. 1975 Jul;116(3):285-93. doi: 10.1620/tjem.116.285.

DOI:10.1620/tjem.116.285

Abstract

Acid mucopolysaccharides in mast cell granules were histochemically studied in the lesion of urticaria pigmentosa and in the dermis of normal human skin. Alcian blue and azure A were used to stain mucopolysaccharides. Bromphenol blue was employed for detection of basic proteins. In a further attempt to identify various polyanions, staining was carried out with alcian blue containing various concentrations of electrolytes. Methylation, saponification, and digestion with streptomyces or testicular hyaluronidase, chondroitinase ABC, sialidase, or desoxyribonuclease were also employed. The results obtained are most likely to suggest the presence of hyaluronic acid in mast cell granules.

摘要

对色素性荨麻疹病变部位及正常人皮肤真皮层肥大细胞颗粒中的酸性黏多糖进行了组织化学研究。采用阿尔辛蓝和天青A对黏多糖进行染色。用溴酚蓝检测碱性蛋白。为进一步鉴定各种聚阴离子，用含有不同浓度电解质的阿尔辛蓝进行染色。还采用了甲基化、皂化以及用链霉菌或睾丸透明质酸酶、软骨素酶ABC、唾液酸酶或脱氧核糖核酸酶进行消化处理。所获结果极有可能表明肥大细胞颗粒中存在透明质酸。