Fernández C, Mattsson J G, Bölske G, Levisohn S, Johansson K E
National Veterinary Institute, Uppsala, Sweden.
Res Vet Sci. 1993 Jul;55(1):130-6. doi: 10.1016/0034-5288(93)90047-j.
Mycoplasma gallisepticum and M synoviae are important avian pathogens causing respiratory diseases which result in great economic losses in poultry farming. Two oligonucleotide probes, complementary to the variable region V8 of 16S rRNA from the avian mycoplasmas M gallisepticum and M synoviae have, therefore, been designed and used in direct filter hybridisation experiments. Both probes gave strong hybridisation signals with their homologous targets, whereas no cross-hybridisations were obtained with any of the other avian mycoplasmas tested. It was possible to detect 2-3 x 10(4) mycoplasma organisms by direct filter hybridisation experiments with radiolabelled probes. The probes were also used to analyse several laboratory strains and field isolates of M gallisepticum and M synoviae with complete agreement between the probe technique and the other methods used for species determination. Atypical M gallisepticum strains also gave strong hybridisation signals with the M gallisepticum specific probe.
鸡毒支原体和滑液支原体是引起呼吸道疾病的重要禽病原体，给家禽养殖造成巨大经济损失。因此，设计了两种与禽支原体鸡毒支原体和滑液支原体16S rRNA可变区V8互补的寡核苷酸探针，并用于直接滤膜杂交实验。两种探针与其同源靶标均产生强烈的杂交信号，而与所测试的任何其他禽支原体均未获得交叉杂交信号。通过用放射性标记探针进行直接滤膜杂交实验，可以检测到2-3×10⁴个支原体生物体。这些探针还用于分析鸡毒支原体和滑液支原体的几种实验室菌株和田间分离株，探针技术与用于物种鉴定的其他方法完全一致。非典型鸡毒支原体菌株与鸡毒支原体特异性探针也产生强烈的杂交信号。
