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[用于下肢动脉硬化闭塞症患者输血的自体血浆的储存与制备]

[The storage and preparation of autologous plasma for transfusions to patients with arteriosclerosis obliterans of the vessels of the lower extremities].

作者信息

Gurevich K Ia, Gendel' L L, Belotserkovskiĭ M V, Strashnov V I, Derushova T V

出版信息

Vestn Khir Im I I Grek. 1993 Mar-Apr;150(3-4):81-4.

PMID:8379092
Abstract

An investigation of the regimen of storage and sorption of plasma in patients with obliterating atherosclerosis of lower extremity vessels has shown that the optimum regimen of storage of plasma is freezing (with addition of 1 thousand of heparin units per 100 ml of plasma) at t = -20 degrees C followed by thawing at t = +6(+/- 2) degrees C in running water before sorption. The optimal sorbent for sorption of plasma of patients with obliterating atherosclerosis of lower extremity vessels is "Actilen" charcoal. The optimal method of sorption is sorption after thawing the plasma. The sufficient degree of plasma purification can be reached in the ratio sorbent/sorbate for charcoal "Actilen" equal to 1:100 and a single perfusion of plasma through the column.

摘要

对下肢血管闭塞性动脉粥样硬化患者血浆储存和吸附方案的研究表明,血浆的最佳储存方案是在t = -20℃下冷冻(每100 ml血浆添加1000单位肝素),然后在吸附前于流动水中在t = +6(±2)℃解冻。用于吸附下肢血管闭塞性动脉粥样硬化患者血浆的最佳吸附剂是“Actilen”活性炭。最佳吸附方法是血浆解冻后进行吸附。对于“Actilen”活性炭,吸附剂/被吸附物比例为1:100且血浆单次通过柱灌注时,可达到足够的血浆纯化程度。

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