Böhme H
Eur J Biochem. 1977 Jan;72(2):283-9. doi: 10.1111/j.1432-1033.1977.tb11251.x.
Antibodies prepared against purified spinach ferredoxin and ferredoxin-NADP+ reductase were used as specific inhibitors of electron-transfer reactions dependent on either ferredoxin or ferredoxin-NADP+ reductase; The possible role of both electron carriers in cyclic electron transport was checked using cytochrome b6 photoreactions as indicator. It could be demonstrated that the ferredoxin antibody inhibits cytochrome b6 photoreduction. Ferredoxin-NADP+ reductase, however, appears not to be involved in this pathway: reductase antibody in concentrations sufficient to completely inhibit electron transport to NADP+ had no effect on cytochrome b6 photoreduction. Quantitative treatment of the immunoassay data showed that osmotically shocked chloroplasts contain both bound ferredoxin and ferredoxin-NADP+ reductase in concentration approximately equal to that of cytochrome b6.
针对纯化的菠菜铁氧化还原蛋白和铁氧化还原蛋白-NADP⁺还原酶制备的抗体，被用作依赖于铁氧化还原蛋白或铁氧化还原蛋白-NADP⁺还原酶的电子传递反应的特异性抑制剂；使用细胞色素b6光反应作为指标，检查了这两种电子载体在循环电子传递中的可能作用。可以证明，铁氧化还原蛋白抗体抑制细胞色素b6光还原。然而，铁氧化还原蛋白-NADP⁺还原酶似乎不参与这条途径：浓度足以完全抑制向NADP⁺的电子传递的还原酶抗体，对细胞色素b6光还原没有影响。免疫测定数据的定量处理表明，经渗透压休克处理的叶绿体中结合态的铁氧化还原蛋白和铁氧化还原蛋白-NADP⁺还原酶的浓度，大约与细胞色素b6的浓度相等。
