Red'ko T P, Shmeleva V L, Ivanov B N, Mukhin E N
Biokhimiia. 1982 Oct;47(10):1695-9.
The effects of ferredoxin concentrations on the correlation between pseudocyclic and non-cyclic electron transport in isolated pea chloroplasts with NADP+ as an electron acceptor was studied. The correlation of psuedocyclic and non-cyclic electron transport upon photoreduction of NADP+ in vitro can be regulated by ferredoxin concentration. At ferredoxin concentrations studied an addition of NADP+ to the system which makes possible electron transfer from ferredoxin to ferredoxin-NADP+-reductase increases the rate of overall electron transport across the chain. Unlike the pseudocyclic electron transport, photoreduction of NADP+ reaches its maximal saturation at relatively low (10-15 microM) concentrations of ferredoxin (Km = approximately 2-5 microM).
研究了铁氧还蛋白浓度对以NADP⁺作为电子受体的离体豌豆叶绿体中假循环和非循环电子传递之间相关性的影响。体外NADP⁺光还原时假循环和非循环电子传递的相关性可受铁氧还蛋白浓度调节。在所研究的铁氧还蛋白浓度下,向体系中添加NADP⁺使电子从铁氧还蛋白转移至铁氧还蛋白-NADP⁺还原酶成为可能,从而提高了整个电子传递链的电子传递速率。与假循环电子传递不同,NADP⁺的光还原在相对较低(10 - 15微摩尔)的铁氧还蛋白浓度(Km约为2 - 5微摩尔)时达到最大饱和度。