Billings-Gagliardi S, Pockwinse S M, Schneider G B
Am J Anat. 1979 Feb;154(2):267-76. doi: 10.1002/aja.1001540210.
Surface coats can be demonstrated on human peripheral blood lymphocytes by staining with ruthenium red, alcian blue, Thorotrast, and cationized ferritin, which are similar in distribution to a 40- to 65-nm layer of amorphous extracellular material recently reported on fixed, freeze-dried lymphocytes. Several additional lines of evidence, including X-ray microanalysis, suggest that the latter is not a contaminant added by freeze-drying. Freeze-drying may provide the means for a morphological assessment of the lymphocyte surface, including the extracellular coat, which may give additional insight into the immune response.
通过用钌红、阿尔辛蓝、氧化钍胶体和阳离子铁蛋白染色，可以在人外周血淋巴细胞上显示表面被膜，其分布类似于最近在固定的、冻干的淋巴细胞上报道的40至65纳米厚的无定形细胞外物质层。包括X射线微量分析在内的其他几条证据表明，后者不是冻干过程中添加的污染物。冻干可能为淋巴细胞表面（包括细胞外被膜）的形态学评估提供手段，这可能会为免疫反应提供更多的见解。
