Korolev E V, Snigirevskaya E S
Acta Histochem Suppl. 1981;23:275-83.
By the changes introduced in the standard procedures of the freeze-drying method (fixation, transportation of specimens from a vacuum apparatus into glass capillary tubes, and embedding) we have managed to simplify the method and to increase the reliability of the results. Fixation of the tissue was carried out after freeze-drying in molecular beams of OsO4 or in an OsO4 solution in carbon tetrachloride. The samples were embedded in araldite through mixtures of carbon tetrachloride and araldite. Membranes of myelin and gregarine pellicle were investigated. The repeat period of myelin at fixation of nerves after freeze-drying in OsO4 molecular beams is 11.2 +/- 0.5 nm and in an OsO4 solution in carbon tetrachloride, 12 +/- 0.4 nm. A tetragonal reticulum was revealed on the plasma-membrane extracellular surface of gregarine which had been prepared by freeze-drying. This type of structure is never observed after the routine fixation in water, while an analogous structure is revealed after freeze-etching.
通过对冷冻干燥法标准程序（固定、将标本从真空装置转移至玻璃毛细管以及包埋）进行改进，我们成功简化了该方法并提高了结果的可靠性。组织固定在OsO4分子束中冷冻干燥后进行，或在四氯化碳中的OsO4溶液中进行。样品通过四氯化碳和阿拉地特的混合物包埋在阿拉地特中。对髓鞘膜和簇虫pellicle进行了研究。在OsO4分子束中冷冻干燥后固定神经时，髓鞘的重复周期为11.2 +/- 0.5纳米，在四氯化碳中的OsO4溶液中为12 +/- 0.4纳米。在通过冷冻干燥制备的簇虫质膜细胞外表面发现了四方网状结构。在常规水固定后从未观察到这种结构类型，而在冷冻蚀刻后发现了类似结构。
