Verweij W R, Namavar F, Schouten W F, Kostense P J, Pellenkoft M, De Graaff J, MacClaren D M
Vrije Universiteit, School of Medicine, Department of Medical Microbiology, Amsterdam, The Netherlands.
J Med Microbiol. 1993 Jan;38(1):13-8. doi: 10.1099/00222615-38-1-13.
Peritoneal cells from rats infected intraperitoneally with Escherichia coli and Bacteroides fragilis, alone or in combination were examined in vitro. Cells were harvested 6 h after implantation of fibrin clots infected with E. coli or B. fragilis, separately or containing both species, and assayed for their bactericidal capacities, chemiluminescence and production of cidal metabolites. Peritoneal cell populations from rats with implants of any of the infected clots showed similar distribution of different subpopulations. Bactericidal activity of peritoneal cells did not differ with the bacterial species used. Chemiluminescence values of peritoneal cells from rats with mono-infected B. fragilis or mixed-infected implanted clots, after stimulation with either particles or chemical stimuli, were significantly higher than those of rats with mono-infected E. coli or sterile clots. The same tendency was seen with regard to the production of cidal metabolites such as hydrogen peroxide and superoxide anions although no significant differences were found.
对经腹腔感染大肠杆菌和脆弱拟杆菌单独感染或联合感染的大鼠的腹膜细胞进行了体外研究。在植入分别感染大肠杆菌或脆弱拟杆菌或同时含有这两种菌的纤维蛋白凝块6小时后收集细胞，并检测其杀菌能力、化学发光和杀菌代谢产物的产生。植入任何一种感染凝块的大鼠的腹膜细胞群体显示出不同亚群的相似分布。腹膜细胞的杀菌活性不因所使用的细菌种类而异。在受到颗粒或化学刺激后，来自脆弱拟杆菌单感染或混合感染植入凝块的大鼠的腹膜细胞的化学发光值显著高于来自大肠杆菌单感染或无菌凝块的大鼠。尽管未发现显著差异，但在过氧化氢和超氧阴离子等杀菌代谢产物的产生方面也观察到了相同的趋势。
