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人类基因组序列的引入使CHO-K1细胞易受嗜双性逆转录病毒感染。

Introduction of human genomic sequences renders CHO-K1 cells susceptible to infection by amphotropic retroviruses.

作者信息

Eglitis M A, Kadan M J, Wonilowicz E, Gould L

机构信息

Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, Maryland 20892.

出版信息

J Virol. 1993 Feb;67(2):1100-4. doi: 10.1128/JVI.67.2.1100-1104.1993.

DOI:10.1128/JVI.67.2.1100-1104.1993
PMID:8380456
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC237468/
Abstract

To learn more about the nature of the block to infection by amphotropic retroviruses exhibited by Chinese hamster cells (CHO-K1), CHO-K1 cells were made susceptible to amphotropic retrovirus infection by introducing genomic DNA from infectable human cells. A clone, designated CHO18, was obtained and shown to be infected as efficiently as NIH 3T3 fibroblasts. Susceptibility of CHO18 cells to infection was specific to retroviruses and vectors bearing an amphotropic envelope. By comparison to CHO-K1 cells, CHO18 cells may provide a useful model for analysis of the molecular events involved in the retrovirus-receptor interaction.

摘要

为了更深入了解中国仓鼠细胞（CHO-K1）对嗜异性逆转录病毒感染的阻断特性，通过导入可感染人类细胞的基因组DNA，使CHO-K1细胞对嗜异性逆转录病毒感染变得敏感。获得了一个名为CHO18的克隆，结果显示其感染效率与NIH 3T3成纤维细胞相同。CHO18细胞对感染的敏感性具有逆转录病毒特异性，并且对携带嗜异性包膜的载体敏感。与CHO-K1细胞相比，CHO18细胞可能为分析逆转录病毒-受体相互作用中涉及的分子事件提供一个有用的模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9870/237468/e2a00446e2d4/jvirol00023-0489-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9870/237468/6784c2a800c1/jvirol00023-0488-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9870/237468/e2a00446e2d4/jvirol00023-0489-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9870/237468/6784c2a800c1/jvirol00023-0488-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9870/237468/e2a00446e2d4/jvirol00023-0489-a.jpg

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本文引用的文献

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Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos.利用重组逆转录病毒研究小鼠胚胎植入后细胞谱系。
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A putative murine ecotropic retrovirus receptor gene encodes a multiple membrane-spanning protein and confers susceptibility to virus infection.一个假定的小鼠嗜亲性逆转录病毒受体基因编码一种多次跨膜蛋白，并赋予对病毒感染的易感性。
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Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line.先进的哺乳动物基因转移：具有多种药物选择标记的高滴度逆转录病毒载体及互补的无辅助病毒包装细胞系。
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Characterization of a human gene conferring sensitivity to infection by gibbon ape leukemia virus.赋予人类对长臂猿白血病病毒感染敏感性的一个基因的特征分析。
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