Gros C, Flecheux O, Dray F
Ann Biol Clin (Paris). 1978;36(4):393-6.
The authors present a reliable and sensitive estimation of progesterone using a conjugate progesterone IIalpha-hémimaleate-beta galactosidase to identify the immunological reaction, the antibodies being obtained from the rabbit by means of a progesterone 11alpha-hemisuccinate bovine serum albumin. The free and bound fractions are separated by addition of rabbit antigamma globulin antibodies bound on glass beads by glutaraldehyde. This method is applied to estimations of cortisol and oestrone.
作者们利用共轭孕酮IIα-半马来酸酯-β-半乳糖苷酶来识别免疫反应，通过孕酮11α-半琥珀酸牛血清白蛋白从兔子身上获取抗体，从而对孕酮进行可靠且灵敏的测定。游离部分和结合部分通过加入用戊二醛结合在玻璃珠上的兔抗γ球蛋白抗体来分离。该方法应用于皮质醇和雌酮的测定。
