Boyle M J, Vasak E, Tschuchnigg M, Turner J J, Sculley T, Penny R, Cooper D A, Tindall B, Sewell W A
Centre for Immunology, St Vincent's Hospital, Sydney, NSW, Australia.
Blood. 1993 Jan 15;81(2):468-74.
Epstein-Barr virus (EBV) has been associated with Hodgkin's disease (HD) in up to 50% of cases, but the subtype of EBV involved has only recently been studied. In this report, biopsy samples from 30 patients with HD were assessed for EBV sequences using both the polymerase chain reaction (PCR) and in situ hybridization (ISH). EBV sequences were localized to the malignant Reed-Sternberg cells and their mononuclear variants (Hodgkin's cells) in 9 of the 30 cases, with 7 demonstrating A-type and 2 B-type EBV sequences. Both of the patients with B-type EBV-associated HD had features to suggest pre-existing immune compromise: one was infected with human immunodeficiency virus (HIV) and had severe CD4+ T-lymphocyte depletion; the other was a debilitated elderly patient with dementia. A previous study suggested that A-type EBV alone is associated with HD and the finding of predominantly A-type EBV in the present series is in keeping with this report. The presence of B-type EBV in the HD of patients with pre-existing immunodeficiency, taken together with the recent report that B-type EBV occurs in HIV-associated non-Hodgkin's lymphoma, suggests that B-type EBV may be an important human pathogen in immunocompromised patients.
爱泼斯坦-巴尔病毒（EBV）在高达50%的霍奇金淋巴瘤（HD）病例中被发现与该病有关，但涉及的EBV亚型直到最近才得到研究。在本报告中，对30例HD患者的活检样本使用聚合酶链反应（PCR）和原位杂交（ISH）技术评估EBV序列。在30例病例中的9例中，EBV序列定位于恶性里德-斯腾伯格细胞及其单核变体（霍奇金细胞），其中7例显示为A型EBV序列，2例为B型EBV序列。两名B型EBV相关HD患者均有提示既往免疫功能受损的特征：一名感染了人类免疫缺陷病毒（HIV），CD4+T淋巴细胞严重耗竭；另一名是患有痴呆症的体弱老年患者。先前的一项研究表明，仅A型EBV与HD有关，本系列研究中主要为A型EBV的发现与该报告一致。在既往免疫功能受损患者的HD中存在B型EBV，再加上最近关于B型EBV出现在HIV相关非霍奇金淋巴瘤中的报告，提示B型EBV可能是免疫功能低下患者的一种重要人类病原体。
