Flamant F, Aubert D, Legrand C, Cosset F L, Samarut J
Laboratoire de Biologie Moléculaire et Cellulaire, INRA-CNRS UMR 49, France.
J Gen Virol. 1993 Jan;74 ( Pt 1):39-46. doi: 10.1099/0022-1317-74-1-39.
Avian leukosis virus-derived vectors were constructed with an internal transcriptional promoter and various 3' non-coding sequences. Deletions were introduced into the downstream U3 long terminal repeat (LTR) to obtain self-inactivation of LTR-mediated transcription after one round of replication. However, 3' non-coding sequences appeared to determine not only self-inactivation of the vectors but also gene transfer efficiency. Further analysis revealed the influence of these sequences on both internal gene expression and RNA packaging. One construct permitted gene transfer while inactivating 5' LTR-promoted transcription.
禽白血病病毒衍生载体是利用内部转录启动子和各种3'非编码序列构建而成的。在下游U3长末端重复序列（LTR）中引入缺失，以便在一轮复制后实现LTR介导转录的自我失活。然而，3'非编码序列似乎不仅决定了载体的自我失活，还决定了基因转移效率。进一步分析揭示了这些序列对内部基因表达和RNA包装的影响。一种构建体允许基因转移，同时使5' LTR启动的转录失活。
