Cosset F L, Girod A, Flamant F, Drynda A, Ronfort C, Valsesia S, Molina R M, Faure C, Nigon V M, Verdier G
Centre de Génétique Moléculaire et Cellulaire, CNRS UMR106, INRA, Université Claude Bernard Lyon-I, Villeurbanne, France.
Virology. 1993 Mar;193(1):385-95. doi: 10.1006/viro.1993.1135.
We have recently described Avian Leukosis Virus (ALV)-based packaging cell lines that can produce helper-free ALV-based retrovirus vectors with A, B, C, and E envelope host ranges. Here, we report that lacZ retroviral vectors of subgroup C or E can infect helper cells of subgroup A (Isolde) which are then able to produce high titers of lacZ recombinant viruses of subgroup A. Superinfection of helper cells by lacZ recombinant virus was performed by cocultivating packaging cells with two subgroup specificities (A and E), but this did not result in increased recombinant virus titers. This "ping-pong" process caused the emergence of replication-competent (RC) viruses which are shown to result from recombination between the viral sequences of the helper cell lines and the cis-acting sequences of the lacZ recombinant virus.
我们最近描述了基于禽白血病病毒（ALV）的包装细胞系，该细胞系能够产生具有A、B、C和E包膜宿主范围的无辅助病毒的基于ALV的逆转录病毒载体。在此，我们报告C或E亚群的lacZ逆转录病毒载体可感染A亚群的辅助细胞（伊索尔德），这些辅助细胞随后能够产生高滴度的A亚群lacZ重组病毒。通过将具有两种亚群特异性（A和E）的包装细胞共同培养，对辅助细胞进行重组病毒的超感染，但这并未导致重组病毒滴度增加。这种“乒乓”过程导致了复制能力（RC）病毒的出现，这些病毒被证明是由辅助细胞系的病毒序列与lacZ重组病毒的顺式作用序列之间的重组产生的。
