Ogawa R, Calvert J G, Yanagida N, Nazerian K
USDA-Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823.
J Gen Virol. 1993 Jan;74 ( Pt 1):55-64. doi: 10.1099/0022-1317-74-1-55.
Insertion of the Escherichia coli lacZ gene into a ClaI restriction enzyme site of a 5.7 kb HindIII fragment of the fowlpox virus (FPV) genome resulted in the generation of stable recombinants. These recombinants produced plaques that were significantly smaller than those produced by parental FPV or by FPV recombinants containing the lacZ gene at other non-essential sites. Insertion of foreign DNA into the ClaI site disrupts a previously unidentified open reading frame (ORF) which potentially encodes a 74K polypeptide. The predicted amino acid sequence of this FPV ORF has 24% identity with the F12L ORF of vaccinia virus, the function of which is not currently known. Production of intracellular FPV was similar in cells infected with recombinant or parental viruses, but the number of infectious extracellular virions released into the medium by the recombinant was about 20% of that released by the parental virus. Likewise, the release of FPV particles, which were labelled in vivo with [3H]thymidine, was significantly lower in recombinant FPV-infected cells. These results suggest that the FPV homologue of the vaccinia virus F12L ORF is involved in the envelopment or release of infectious extracellular virions.
将大肠杆菌lacZ基因插入禽痘病毒（FPV）基因组5.7 kb HindIII片段的ClaI限制性酶切位点，产生了稳定的重组体。这些重组体产生的噬菌斑明显小于亲本FPV或在其他非必需位点含有lacZ基因的FPV重组体产生的噬菌斑。将外源DNA插入ClaI位点会破坏一个先前未鉴定的开放阅读框（ORF），该开放阅读框可能编码一种74K多肽。该FPV ORF的预测氨基酸序列与痘苗病毒的F12L ORF有24%的同源性，其功能目前尚不清楚。感染重组病毒或亲本病毒的细胞中细胞内FPV的产生情况相似，但重组体释放到培养基中的有感染性的细胞外病毒粒子数量约为亲本病毒释放量的20%。同样，在体内用[3H]胸苷标记的FPV颗粒在重组FPV感染的细胞中的释放也明显较低。这些结果表明，痘苗病毒F12L ORF的FPV同源物参与了有感染性的细胞外病毒粒子的包裹或释放。
