Maruta K, Ichimura K, Matsui H, Yamagami T, Sano A, Tsuji H
Department of Orthopaedic Surgery, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
J Orthop Res. 1993 Jan;11(1):92-103. doi: 10.1002/jor.1100110111.
To examine the presence of substances which inhibit calcification in human ligamentum flavum, the inhibitory effect of an Na2HPO4 extract of the flavum was determined in terms of the in vitro calcium uptake of the ligamentum flavum matrix. Additionally, grafts of extracted and non-extracted dry ligamentum flavum matrices were transplanted into the dorsal muscles of rats, and calcification in the grafts was examined radiologically and histochemically. In order to determine if component cells of human ligamentum flavum produce calcification inhibitors, ligamentum flavum cells were cultured, and the crystal inhibitor activity of the culture medium was measured by a seed test which used hydroxyapatite as the nucleus of precipitation. The calcification reaction system demonstrated that the ligamentum flavum extract contains an inhibitory factor for calcium uptake by the ligamentum flavum matrix. The seed test revealed that human ligamentum flavum cells produce calcification inhibitor activity.
为检测人黄韧带中抑制钙化物质的存在情况，根据黄韧带基质的体外钙摄取情况测定了黄韧带的Na2HPO4提取物的抑制作用。此外，将提取和未提取的干燥黄韧带基质移植到大鼠背部肌肉中，通过放射学和组织化学方法检查移植物中的钙化情况。为了确定人黄韧带的组成细胞是否产生钙化抑制剂，培养了黄韧带细胞，并通过以羟基磷灰石作为沉淀核的种子试验测量培养基的晶体抑制剂活性。钙化反应系统表明，黄韧带提取物含有抑制黄韧带基质摄取钙的因子。种子试验显示，人黄韧带细胞产生钙化抑制剂活性。
