编码高亲和力人神经降压素受体的互补DNA的克隆与表达
Cloning and expression of a complementary DNA encoding a high affinity human neurotensin receptor.
作者信息
Vita N, Laurent P, Lefort S, Chalon P, Dumont X, Kaghad M, Gully D, Le Fur G, Ferrara P, Caput D
机构信息
Unité Biochimie des Protéines, Sanofi Elf Biorecherches, Labège, France.
出版信息
FEBS Lett. 1993 Feb 8;317(1-2):139-42. doi: 10.1016/0014-5793(93)81509-x.
A human neurotensin receptor (hNTR) cDNA was cloned from the colonic adenocarcinoma cell line HT29. The cloned cDNA encodes a putative peptide of 418 amino acids with 7 transmembrane domains. The amino acid sequence of the hNTR is 84% identical to the rat NTR [Neuron, 4 (1990) 847-854]. Transfection of this cDNA into COS cells results in the expression of receptors with pharmacological properties similar to those found with HT29 cells. Northern blot analysis using the hNTR cDNA probe indicated a single transcript of 4 kb in the brain, the small intestine and blood mononuclear cells.
从结肠腺癌细胞系HT29中克隆出人类神经降压素受体（hNTR）cDNA。克隆出的cDNA编码一个推定的含418个氨基酸且具有7个跨膜结构域的肽段。hNTR的氨基酸序列与大鼠NTR的氨基酸序列有84%的同源性[《神经元》，4 (1990) 847 - 854]。将此cDNA转染到COS细胞中可导致具有与HT29细胞中发现的药理特性相似的受体表达。使用hNTR cDNA探针进行的Northern印迹分析表明，在脑、小肠和血液单核细胞中有一个4 kb的单一转录本。
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