Bellini T, Tomasi M, Dallocchio F
Istituto di Chimica Biologica, Università di Ferrara, Italy.
Biochim Biophys Acta. 1993 Feb 13;1161(2-3):323-7. doi: 10.1016/0167-4838(93)90232-g.
Incubation of Sendai virus with pyridoxal 5'-phosphate (PLP) causes inhibition of hemolytic activity, a slight reduction of hemagglutinating activity, and an increase in neuraminidase activity. The effects on hemagglutination and neuraminidase are prevented by the presence in the incubation mixture of sialyl lactose, a substrate of hemagglutinin-neuraminidase. Incubation with PLP of the water-soluble enzymatic domain of the neuraminidase has no effect on enzymatic activity, while the allosteric inhibition (Dallocchio et al. (1991) Biochem. Int. 25, 663-668) disappears. Both virus-bound and solubilized neuraminidase are selectively modified by PLP at the lysine-553. Our data suggest that PLP inactivates a previously undetected inhibitory site on the viral neuraminidase, and that a physiological effector is present on the viral envelope.
仙台病毒与磷酸吡哆醛（PLP）一起温育会导致溶血活性受到抑制、血凝活性略有降低以及神经氨酸酶活性增加。血凝素 - 神经氨酸酶的底物唾液酸乳糖存在于温育混合物中时，可防止对血凝和神经氨酸酶的影响。神经氨酸酶的水溶性酶结构域与PLP温育对酶活性没有影响，而异构抑制（Dallocchio等人（1991年），《生物化学国际》25卷，663 - 668页）消失。病毒结合的和可溶解的神经氨酸酶在赖氨酸 - 553处都被PLP选择性修饰。我们的数据表明，PLP使病毒神经氨酸酶上一个先前未被检测到的抑制位点失活，并且病毒包膜上存在一种生理效应物。
