Kumar S, Peña L A, de Vellis J
Laboratory of Biomedical and Environmental Sciences, University of California, Los Angeles 90024-1759.
Brain Res Mol Brain Res. 1993 Jan;17(1-2):163-8. doi: 10.1016/0169-328x(93)90086-5.
Normal CNS glial cells manufacture neurotrophin receptors and are competent to respond to NGF. Neurotrophins bind a common receptor (LNGFR) and ligand-specific, tyrosine kinase-containing subunits (TrkA, TrkB, or TrkC). Northern blots and transcription assays reveal complex transcriptional regulation of LNGFR in astrocytes; from undetectable basal levels, NGF dramatically induces LNGFR within 4-6 h. Oligodendrocytes' relatively high basal levels are unaffected by NGF. TrkA mRNA was undetectable, however, TrkB was present and upregulated by NGF in astrocytes but not oligodendrocytes. The results are consistent with receptor autoregulation by its ligand and suggest that NGF plays a role in normal glial functions.
正常的中枢神经系统神经胶质细胞制造神经营养因子受体并能够对神经生长因子（NGF）作出反应。神经营养因子与一种共同受体（低亲和力神经生长因子受体，LNGFR）以及配体特异性的含酪氨酸激酶亚基（TrkA、TrkB或TrkC）结合。Northern印迹法和转录分析揭示了星形胶质细胞中LNGFR的复杂转录调控；从无法检测到的基础水平开始，NGF在4-6小时内显著诱导LNGFR。少突胶质细胞相对较高的基础水平不受NGF影响。然而，TrkA mRNA无法检测到，TrkB存在且在星形胶质细胞中被NGF上调，但在少突胶质细胞中未上调。这些结果与受体被其配体的自身调节一致，并表明NGF在正常神经胶质功能中发挥作用。
