神经营养因子-3上调中枢神经系统胶质细胞系中的神经生长因子受体。

Neurotrophin-3 upregulates NGF receptors in a central nervous system glial cell line.

作者信息

Spoerri P E, Romanello S, Petrelli L, Negro A, Guidolin D, Skaper S D

机构信息

Fidia Research Laboratories, Fidia S.p.A., Abano Terme, Italy.

出版信息

Neuroreport. 1993 Jan;4(1):33-6. doi: 10.1097/00001756-199301000-00008.

DOI:10.1097/00001756-199301000-00008

PMID:8453034

Abstract

The low-affinity nerve growth factor (NGF) receptor (LNGFR) binds the neurotrophins NGF, brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) with similar affinities. Here we report on the ability of NT-3 to regulate the expression of the LNGFR in C6 glioma cells. LNGFR-like immunoreactivity (LNGFR-IR) was examined in C6 cells treated for 16 h with NT-3 and exposed to the antibody 192-IgG followed by immunoglobulins conjugated with colloidal gold by means of ultrastructural morphometric analysis. Untreated C6 cells exhibited some positive LNGFR-IR, while C6 cells treated with NT-3 displayed significantly increased (2.3 fold) LNGFR-IR. The increase in LNGFR protein was accompanied by a greater quantity of LNGFR mRNA in NT-3-treated cells. Thus, LNGFR can be upregulated by the structurally related neurotrophin NT-3.

摘要

低亲和力神经生长因子（NGF）受体（LNGFR）以相似的亲和力结合神经营养因子NGF、脑源性神经营养因子（BDNF）和神经营养因子-3（NT-3）。在此，我们报告NT-3调节C6胶质瘤细胞中LNGFR表达的能力。通过超微结构形态计量分析，在用NT-3处理16小时并暴露于抗体192-IgG，随后用与胶体金偶联的免疫球蛋白处理的C6细胞中检测LNGFR样免疫反应性（LNGFR-IR）。未处理的C6细胞表现出一些阳性LNGFR-IR，而用NT-3处理的C6细胞显示LNGFR-IR显著增加（2.3倍）。在NT-3处理的细胞中，LNGFR蛋白的增加伴随着更多数量的LNGFR mRNA。因此，结构相关的神经营养因子NT-3可上调LNGFR。