人精液核糖核酸酶。定位、特性以及被十二烷基硫酸钠、硫酸锌和乙二胺四乙酸抑制的情况。

Human semen ribonuclease. Location, properties and inhibition by sodium dodecyl sulfate, zinc sulfate and EDTA.

作者信息

Mujica A, Romero G, Hernandez-Montes H

出版信息

Int J Fertil. 1976(2):109-13.

Abstract

Optimal conditions were established for RNase activity measurement. The enzyme was measured in human seminal plasma as well as in spermatozoa. Results suggest that sperm enzyme may come from seminal plasma contamination and that RNase may be used as a marker enzyme for seminal plasma contamination. Sodium dodecylsulfate, a reagent utilized to produce the solubilization of the spermatozoa, produced a very strong inhibition of the RNase at low concentrations (530 muM). Zinc sulfate and EDTA also produced inhibition of the RNase activity. Such inhibition may be very useful in future studies of RNA metabolism in human spermatozoa.

摘要

确定了测量核糖核酸酶（RNase）活性的最佳条件。在人类精浆以及精子中对该酶进行了检测。结果表明，精子中的酶可能来自精浆污染，并且核糖核酸酶可用作精浆污染的标记酶。十二烷基硫酸钠是一种用于使精子溶解的试剂，在低浓度（530μM）时对核糖核酸酶产生非常强烈的抑制作用。硫酸锌和乙二胺四乙酸（EDTA）也对核糖核酸酶活性产生抑制作用。这种抑制作用在未来人类精子RNA代谢的研究中可能非常有用。