Grant A J, Koski G, Treistman S N
Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545.
Brain Res. 1993 Jan 15;600(2):280-4. doi: 10.1016/0006-8993(93)91384-5.
Undifferentiated pheochromocytoma (PC12) cells were chronically exposed to 200 mM ethanol for six days. Parallel cultures were maintained without ethanol. Cells chronically exposed to ethanol had significantly larger voltage-gated calcium currents than those grown in the absence of ethanol. Concurrent radioisotopic flux assays confirm that calcium influx is, indeed, increased in the chronically treated cells. However, acute exposure to ethanol at the same concentration as those used for the chronic studies greatly reduced the magnitude of the currents and net calcium influx.
未分化嗜铬细胞瘤（PC12）细胞长期暴露于200 mM乙醇中6天。同时设置无乙醇的平行培养物。长期暴露于乙醇的细胞比未接触乙醇生长的细胞具有明显更大的电压门控钙电流。同时进行的放射性同位素通量测定证实，长期处理的细胞中钙内流确实增加。然而，与慢性研究中使用的相同浓度乙醇的急性暴露极大地降低了电流幅度和净钙内流。
