产生对人血清具有抗性的重组逆转录病毒的高滴度包装细胞。
High-titer packaging cells producing recombinant retroviruses resistant to human serum.
作者信息
Cosset F L, Takeuchi Y, Battini J L, Weiss R A, Collins M K
机构信息
Chester Beatty Laboratory, Institute of Cancer Research, London, United Kingdom.
出版信息
J Virol. 1995 Dec;69(12):7430-6. doi: 10.1128/JVI.69.12.7430-7436.1995.
Abstract
Novel retroviral protein expression constructs were designed to retain minimal retroviral sequences and to express dominant selectable markers by reinitiation of translation after expression of the viral genes. HT1080 cells were selected as producer cells for their ability to release high-titer viruses that are resistant to inactivation by human serum. Two HT1080-based packaging cell lines which produce Moloney murine leukemia virus cores with envelope glycoproteins of either amphotropic murine leukemia virus (FLYA13 line) or cat endogenous virus RD114 (FLYRD18 line) are described. Direct comparison with previous retroviral packaging systems indicated that 100-fold-higher titers of helper-free recombinant viruses were released by the FLYA13 and FLYRD18 lines.
摘要
新型逆转录病毒蛋白表达构建体的设计旨在保留最少的逆转录病毒序列，并通过在病毒基因表达后重新起始翻译来表达显性选择标记。选择HT1080细胞作为生产细胞，因为它们能够释放对人血清灭活具有抗性的高滴度病毒。描述了两种基于HT1080的包装细胞系，它们产生带有双嗜性鼠白血病病毒包膜糖蛋白的莫洛尼鼠白血病病毒核心（FLYA13系）或猫内源性病毒RD114（FLYRD18系）。与先前的逆转录病毒包装系统直接比较表明，FLYA13和FLYRD18系释放的无辅助重组病毒滴度高100倍。
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