大麦液泡膜质子转运无机焦磷酸酶cDNA的分子克隆
Molecular cloning of cDNA for vacuolar membrane proton-translocating inorganic pyrophosphatase in Hordeum vulgare.
作者信息
Tanaka Y, Chiba K, Maeda M, Maeshima M
机构信息
Chemical Tolerance, National Institute of Agrobiological Resources, Ibaraki, Japan.
出版信息
Biochem Biophys Res Commun. 1993 Feb 15;190(3):1110-4. doi: 10.1006/bbrc.1993.1164.
We obtained a cDNA clone(PP10) for the vacuolar membrane proton-translocating inorganic pyrophosphatase from barley roots by immunoscreening. The nucleotide sequence contained a 2308 bp open reading frame capable of coding for a polypeptide with 761 amino acids (M(r) 79,841). The polypeptide is highly hydrophobic and 12 membrane-spanning regions are deduced from the hydropathic evaluation. The characteristic cluster of the basic and acidic residues is observed in the hydrophilic segment. A consensus sequence with the dicyclohexyl-carbodiimide binding subunits of FOF1-type and vacuolar-type H(+)-ATPase is also observed in the membrane-spanning domain 5. Comparison of the deduced amino acid sequence with that of pyrophosphatase cDNA from Arabidopsis thaliana revealed 85.8% homology.
我们通过免疫筛选获得了来自大麦根的液泡膜质子转运无机焦磷酸酶的cDNA克隆（PP10）。核苷酸序列包含一个2308 bp的开放阅读框，能够编码一个含有761个氨基酸（M(r) 79,841）的多肽。该多肽具有高度疏水性，通过亲水性评估推断出12个跨膜区域。在亲水片段中观察到碱性和酸性残基的特征性簇。在跨膜结构域5中也观察到与FOF1型和液泡型H(+) -ATPase的二环己基碳二亚胺结合亚基的共有序列。将推导的氨基酸序列与拟南芥焦磷酸酶cDNA的序列进行比较，发现同源性为85.8%。
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