源自以腹水瘤或实体瘤形式生长的Walker 256癌细胞的质膜的蛋白质和酶含量。

Protein and enzyme content of plasma membranes derived from Walker 256 carcinoma cells grown as ascitic or solid tumours.

作者信息

Clark C M, Goodlad G A

机构信息

School of Biological and Medical Sciences, Division of Cell and Molecular Biology, University of St. Andrews, Fife, Scotland, UK.

出版信息

Cancer Lett. 1993 Feb;68(2-3):91-4. doi: 10.1016/0304-3835(93)90133-t.

DOI:10.1016/0304-3835(93)90133-t

PMID:8383003

Abstract

A preliminary investigation of enzymes functioning at the cell surface indicates that when Walker 256 carcinoma cells growing ascitically are induced to grow as a solid tumour there is a marked increase in the activities of Na+, K(+)-ATPase, Mg(2+)-ATPase, 5'-nucleotidase, alkaline phosphatase and phosphodiesterase I; 5'-nucleotidase and alkaline phosphatase being particularly affected. These enzyme changes occur in the absence of any major alteration in the protein composition of the plasma membrane.

摘要

对在细胞表面起作用的酶的初步研究表明，当腹水生长的Walker 256癌细胞被诱导生长为实体瘤时，Na +、K(+)-ATP酶、Mg(2+)-ATP酶、5'-核苷酸酶、碱性磷酸酶和磷酸二酯酶I的活性显著增加；5'-核苷酸酶和碱性磷酸酶受到的影响尤为明显。这些酶的变化发生在质膜蛋白质组成没有任何重大改变的情况下。