Goodlad G A, Clark C M
School of Biological and Medical Sciences, Division of Cell and Molecular Biology, University of St. Andrews, UK.
Biochim Biophys Acta. 1993 Jan 18;1145(1):177-9. doi: 10.1016/0005-2736(93)90395-g.
Plasma membranes from Walker 256 carcinoma cells grown ascitically or as a solid tumour were examined with respect to actin content, [3H]cytochalasin B-binding and the binding of 125I-labelled G-actin to membrane proteins separated by SDS-PAGE. Differences were observed both in cytochalasin B-binding to membrane actin and affinity of 125I-labelled G-actin for specific membrane proteins.
对以腹水形式生长或作为实体瘤生长的Walker 256癌细胞的质膜进行了肌动蛋白含量、[3H]细胞松弛素B结合以及125I标记的G-肌动蛋白与通过SDS-PAGE分离的膜蛋白结合方面的检测。在细胞松弛素B与膜肌动蛋白的结合以及125I标记的G-肌动蛋白对特定膜蛋白的亲和力方面均观察到差异。
