Lew A M, Thomas L M, Huntington P J
Walter & Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Parkville, Vic., Australia.
Vet Microbiol. 1993 Jan;34(1):1-5. doi: 10.1016/0378-1135(93)90002-o.
Sera of sixteen horses with clinical signs of EIA from six different outbreaks and sera of 100 uninfected horses were used to validate an ELISA for EIA diagnosis. The antigen used was a recombinant protein derived from the amino-terminal portion of the transmembrane envelope protein of EIA (gp45). Reactivity between positive and negative sera could be clearly distinguished. Comparison with the traditional agar gel immunodiffusion test (commonly called the Coggins test) showed that the ELISA was superior in sensitivity. Comparison of this ELISA with the FAST-ELISA system showed that the latter was less sensitive. Although the FAST-ELISA was much faster to perform, it could not be recommended as a diagnostic test in its present form, because the margin between reactivity by a positive serum and a negative serum was not high.
来自6起不同疫情且有马传染性贫血（EIA）临床症状的16匹马的血清，以及100匹未感染马的血清，被用于验证一种用于EIA诊断的酶联免疫吸附测定（ELISA）。所使用的抗原是一种源自EIA跨膜包膜蛋白（gp45）氨基末端部分的重组蛋白。阳性和阴性血清之间的反应性能够被清楚地区分。与传统的琼脂凝胶免疫扩散试验（通常称为科金斯试验）比较表明，该ELISA在敏感性方面更具优势。将这种ELISA与快速ELISA系统比较表明，后者敏感性较低。尽管快速ELISA执行起来要快得多，但因其目前的形式下阳性血清和阴性血清的反应性差异不大，故不能推荐将其作为诊断试验。
