Gilles C, Piette J, Rombouts S, Laurent C, Foidart J M
Laboratory of General Biology, University of Liège, Belgium.
Int J Cancer. 1993 Mar 12;53(5):872-9. doi: 10.1002/ijc.2910530527.
Ten immortalized cell lines were established by transfection of human cervical keratinocytes (CK) with HPV-33 DNA and some of their characteristics were investigated. The following observations were made: (a) several cell lines have reached over 100 population doublings in vitro; (b) 3 transcripts were observed, 2 being encoded by the E6/E7 open reading frames (ORFs); (c) cytogenetic analyses showed important genetic modifications such as aneuploidy and isochromosome formation of the q arm of chromosome 8; (d) 2 of the 10 cell lines developed colonies in soft agar but none was able to form tumors when injected s.c. into nude mice; (e) Southern analysis suggested that a single copy of HPV-33 is integrated at a single common site within the genome of the 10 cell lines. These immortalized cell lines should be useful for studying mechanisms involved in proliferation, differentiation and neoplastic transformation of CK by HPV-33.
通过用HPV - 33 DNA转染人宫颈角质形成细胞（CK）建立了10个永生化细胞系，并对其一些特性进行了研究。得到了以下观察结果：(a) 几个细胞系在体外已达到超过100次群体倍增；(b) 观察到3种转录本，其中2种由E6/E7开放阅读框（ORF）编码；(c) 细胞遗传学分析显示出重要的基因改变，如非整倍体和8号染色体q臂的等臂染色体形成；(d) 10个细胞系中有2个在软琼脂中形成集落，但皮下注射到裸鼠体内时均不能形成肿瘤；(e) Southern分析表明，HPV - 33的单拷贝整合在10个细胞系基因组内的一个单一共同位点。这些永生化细胞系对于研究HPV - 33参与CK增殖、分化和肿瘤转化的机制应该是有用的。
