Atke A, Sjøholm P, Vilhardt H
Department of Medical Physiology C, University of Copenhagen, Denmark.
J Recept Res. 1993;13(1-4):295-304. doi: 10.3109/10799899309073661.
The dissociation course of the hormone receptor complexes of rat myometrial oxytocin receptors was studied. The dissociation course was biphasic with a major component displaying an almost irreversible binding character (time constant 0.003 min-1). This irreversibility was independent of hormonal status of the myometrium as well as the type of divalent cation present in the medium, and was not caused by endocytosis of the receptor. However, as addition of EDTA caused an immediate dissociation of all receptor binding, the irreversibility must be of a non-covalent character. It is suggested that new analysis methods for receptor kinetics including the possibility of partly irreversible binding mechanisms should be considered.
研究了大鼠子宫肌层催产素受体的激素受体复合物的解离过程。解离过程呈双相性，主要成分显示出几乎不可逆的结合特性（时间常数为0.003分钟-1）。这种不可逆性与子宫肌层的激素状态以及培养基中存在的二价阳离子类型无关，且不是由受体内吞作用引起的。然而，由于加入乙二胺四乙酸（EDTA）会导致所有受体结合立即解离，所以这种不可逆性必定是非共价性质的。建议应考虑用于受体动力学的新分析方法，包括部分不可逆结合机制的可能性。
