Liebl B, Anhäupl T, Haen E, Günster B, Georgieff M
Walther-Straub-Institut für Pharmakologie und Toxikologie, Universität München, Germany.
J Recept Res. 1993;13(1-4):369-78. doi: 10.3109/10799899309073667.
Using a Tecan robotic sample processor and IBM compatible PCs we have developed a flexible, partially automated radioligand binding assay system. It handles pipetting parameters of up to 16 saturation or competition experiments at a time with up to 24 radioligand- or competitor-concentrations in a range over 4 orders of magnitude per experiment. The system provides enough flexibility so that all pipetting parameters including different tube-, rack-sizes, sample volumina and pipetting sequences may be easily adapted to the large variety of experimental requirements in binding assays. It rationalizes and increases assay throughput (up to 70% spare of working time), improves reliance and reproducibility of results. Radioactive exposure is minimized to the time preparing the radioligand working solution and transferring the sample tubes to and from the sample processor. The system has proven effective in various investigations on binding interactions, as well as in clinical studies on receptor expression under physiologic, pathological and therapeutic conditions.
我们使用Tecan机器人样本处理器和IBM兼容个人电脑，开发了一种灵活的、部分自动化的放射性配体结合分析系统。它一次可处理多达16个饱和或竞争实验的移液参数，每个实验有多达24种放射性配体或竞争剂浓度，浓度范围跨越4个数量级。该系统具有足够的灵活性，以至于所有移液参数，包括不同的试管、架子尺寸、样本体积和移液顺序，都可以轻松适应结合分析中各种各样的实验要求。它使分析流程合理化并提高了分析通量（最多可节省70%的工作时间），提高了结果的可靠性和可重复性。放射性暴露被最小化到制备放射性配体工作溶液以及将样本试管移入和移出样本处理器的时间。该系统已在各种结合相互作用研究以及生理、病理和治疗条件下受体表达的临床研究中证明有效。
