McPherson G A
J Pharmacol Methods. 1985 Nov;14(3):213-28. doi: 10.1016/0160-5402(85)90034-8.
It is now well accepted that graphic methods are a poor choice of analytical technique for analyzing radioligand binding data given the complexity of some radioligand-binding sites interactions; this is true not only for studies performed at equilibrium but also for those examining kinetic properties of the radioligand. As a consequence the computer plays an important role in all forms of radioligand binding experiments because it reduces the labor-intensive calculations associated with such experiments and it allows the use of more appropriate weighted nonlinear curve-fitting techniques that more accurately describe the radioligand-binding site interaction. This paper describes a collection of four programs (KINETIC, EBDA, LIGAND, and LOWRY) that can be used to analyze most forms of radioligand binding experiments. The programs have been adapted for use on an IBM PC, which is a popular, inexpensive microcomputer and which is well suited to the analysis of radioligand binding data.
鉴于某些放射性配体结合位点相互作用的复杂性，现在人们普遍认为，图形方法是分析放射性配体结合数据的一种糟糕的分析技术选择；这不仅适用于在平衡状态下进行的研究，也适用于那些研究放射性配体动力学性质的研究。因此，计算机在所有形式的放射性配体结合实验中都发挥着重要作用，因为它减少了与此类实验相关的劳动密集型计算，并且允许使用更合适的加权非线性曲线拟合技术，这些技术能更准确地描述放射性配体结合位点的相互作用。本文描述了四个程序（KINETIC、EBDA、LIGAND和LOWRY）的集合，这些程序可用于分析大多数形式的放射性配体结合实验。这些程序已被改编用于IBM个人计算机，这是一种流行且价格低廉的微型计算机，非常适合分析放射性配体结合数据。
