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黑麦核酸酶I作为研究具有大可变臂的tRNA结构的工具。

Rye nuclease I as a tool for structural studies of tRNAs with large variable arms.

作者信息

el Adlouni C, Keith G, Dirheimer G, Szarkowski J W, Przykorska A

机构信息

Unité Structure des Macromolécules Biologiques et Mécanismes de Reconnaissance, Université Louis Pasteur, Strasbourg, France.

出版信息

Nucleic Acids Res. 1993 Feb 25;21(4):941-7. doi: 10.1093/nar/21.4.941.

DOI:10.1093/nar/21.4.941
PMID:8383845
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC309228/
Abstract

A single-strand-specific nuclease from rye germ (Rn nuclease I) was used for secondary and tertiary structure investigations of tRNAs with large variable arms (class II tRNAs). We have studied the structure in solution of two recently sequenced tRNA(Leu): yeast tRNA(Leu)(ncm5UmAA) and bovine tRNA(Leu)(XmAA) as well as yeast tRNA(Leu)(UAG), tRNA(Leu)(m5CAA) and tRNA(Ser)(IGA). The latter is the only tRNA with a long variable arm for which the secondary and tertiary structure has already been studied by use of chemical probes and computer modelling. The data obtained in this work showed that the general model of class II tRNAs proposed by others for tRNA(Ser) can be extended to tRNAs(Leu) as well. However interesting differences in the structure of tRNAs(Leu) versus tRNA(Ser)(IGA) were also noticed. The main difference was observed in the accessibility of the variable loops to nucleolytic attack of Rn nuclease I: variable loops of all studied tRNA(Leu) species were cut by Rn nuclease I, while that of yeast tRNA(Ser)(IGA) was not. This could be due to differences in stability of the variable arms and the lengths of their loops which are 3 and 4 nucleotides in tRNA(Ser)(IGA) and tRNAs(Leu) respectively.

摘要

来自黑麦胚芽的单链特异性核酸酶（Rn核酸酶I）被用于对具有大可变臂的tRNA（II类tRNA）进行二级和三级结构研究。我们研究了两种最近测序的tRNA(Leu)在溶液中的结构：酵母tRNA(Leu)(ncm5UmAA)和牛tRNA(Leu)(XmAA)，以及酵母tRNA(Leu)(UAG)、tRNA(Leu)(m5CAA)和tRNA(Ser)(IGA)。后者是唯一一种具有长可变臂的tRNA，其二级和三级结构已经通过化学探针和计算机建模进行了研究。这项工作中获得的数据表明，其他人针对tRNA(Ser)提出的II类tRNA的通用模型也可以扩展到tRNA(Leu)。然而，也注意到了tRNA(Leu)与tRNA(Ser)(IGA)在结构上有趣的差异。主要差异在于可变环对Rn核酸酶I的核酸酶攻击的可及性：所有研究的tRNA(Leu)种类的可变环都被Rn核酸酶I切割，而酵母tRNA(Ser)(IGA)的可变环则未被切割。这可能是由于可变臂的稳定性差异以及它们环的长度不同，在tRNA(Ser)(IGA)中为3个核苷酸，在tRNA(Leu)中为4个核苷酸。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/038a/309228/ae9054f0110e/nar00053-0159-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/038a/309228/4ca7a9730fa8/nar00053-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/038a/309228/ae9675238144/nar00053-0156-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/038a/309228/fbb717d9a706/nar00053-0157-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/038a/309228/c03069e69d46/nar00053-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/038a/309228/ae9054f0110e/nar00053-0159-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/038a/309228/4ca7a9730fa8/nar00053-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/038a/309228/ae9675238144/nar00053-0156-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/038a/309228/fbb717d9a706/nar00053-0157-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/038a/309228/c03069e69d46/nar00053-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/038a/309228/ae9054f0110e/nar00053-0159-a.jpg

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本文引用的文献

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Primary structure of three tRNAs from brewer's yeast: tRNAPro2, tRNAHis1 and tRNAHis2.
来自酿酒酵母的三种tRNA的一级结构：tRNAPro2、tRNAHis1和tRNAHis2。
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