Heyman T, Agoutin B, Fix C, Dirheimer G, Keith G
Unité de recherche associée 1342 du CNRS, Institut Curie-Biologie, Orsay, France.
FEBS Lett. 1994 Jun 27;347(2-3):143-6. doi: 10.1016/0014-5793(94)00524-9.
The primary structure of Saccharomyces cerevisiae tRNA(Ser)GCU is presented (EMBL database accession No. X74268 S. cerevisiae tRNA-Ser). In addition, quantitation of the relative amounts of serine isoaccepting tRNAs in yeast grown on different media showed that the minor tRNA(Ser)GCU decreased while the major tRNA(Ser)AGA increased as the growth rate and the cellular protein content increased. The minor species, tRNA(Ser)CGA and tRNA(Ser)UGA, were not separated by our gel system, however, taken together they appeared to vary in the same way as tRNA(Ser)GCU. These data suggest a growth rate dependence of yeast tRNAs similar to that previously described for E. coli tRNAs.
展示了酿酒酵母tRNA(Ser)GCU的一级结构（EMBL数据库登录号：X74268 酿酒酵母tRNA-Ser）。此外，对在不同培养基上生长的酵母中丝氨酸同工受体tRNA相对含量的定量分析表明，随着生长速率和细胞蛋白质含量的增加，次要的tRNA(Ser)GCU减少，而主要的tRNA(Ser)AGA增加。然而，次要种类tRNA(Ser)CGA和tRNA(Ser)UGA未被我们的凝胶系统分离，但总体来看，它们的变化方式与tRNA(Ser)GCU相同。这些数据表明酵母tRNA对生长速率的依赖性与先前描述的大肠杆菌tRNA相似。
