Geller A I
Division of Endocrinology, Children's Hospital, Boston, Massachusetts 02115.
Curr Opin Genet Dev. 1993 Feb;3(1):81-5. doi: 10.1016/s0959-437x(05)80345-4.
Gene transfer into neural cells in the adult mammalian brain using vectors derived from the herpes simplex virus HSV-1 has great promise both for elucidating neuronal physiology and brain mechanisms, and for gene therapy of neurological diseases. Two kinds of HSV-1 vectors are being explored: first, defective HSV-1 vectors are small plasmids containing essential HSV-1 cis-acting functions that use HSV-1 mutants as helper virus for packaging; and second, vectors that contain a recombinant gene inserted into the HSV-1 genome. Recently, several genes that alter neuronal physiology have been expressed from defective HSV-1 vectors, both in cultured neurons and in vivo.
利用源自单纯疱疹病毒1型（HSV-1）的载体将基因导入成年哺乳动物大脑中的神经细胞，对于阐明神经元生理学和脑机制以及神经疾病的基因治疗都具有巨大潜力。目前正在探索两种HSV-1载体：第一种，缺陷型HSV-1载体是含有必需HSV-1顺式作用功能的小质粒，其利用HSV-1突变体作为辅助病毒进行包装；第二种，是将重组基因插入HSV-1基因组的载体。最近，几种改变神经元生理学的基因已通过缺陷型HSV-1载体在培养的神经元和体内表达。
