Vuotto M L, Peluso G, Mancino D, Colonna G, Facchiano A, Ielpo M T, Ravagnan G, Metafora S
Institute of General Pathology, First Medical School, University of Naples, Italy.
J Leukoc Biol. 1993 Mar;53(3):214-22. doi: 10.1002/jlb.53.3.214.
SV-IV, a 9.8-kd protein isolated from rat seminal vesicle secretion, has been shown to have strong non-species-specific immunosuppressive, anti-inflammatory, antithrombotic, and antiphospholipase A2 properties. The present investigation was undertaken to determine the mechanism of action of its immunosuppressive effects. It was found that SV-IV is a potent inhibitor of interleukin-1 (IL-1) release from lipopolysaccharide (LPS)-activated human adherent monocytes and an effective inhibitor of IL-1-induced thymocyte proliferation. The ability of SV-IV to form a noncovalent dimeric association with IL-1 alpha but not with IL-1 beta, its ability to induce a marked decrease of IL-1 binding to its own receptors on the thymocyte surface, and its capacity to bind specifically to the macrophage plasma membrane might play an important role in the molecular mechanism of these inhibitory effects.
SV-IV是一种从大鼠精囊分泌物中分离出的9.8千道尔顿蛋白质，已被证明具有强大的非物种特异性免疫抑制、抗炎、抗血栓形成和抗磷脂酶A2特性。本研究旨在确定其免疫抑制作用的作用机制。研究发现，SV-IV是脂多糖（LPS）激活的人贴壁单核细胞释放白细胞介素-1（IL-1）的强效抑制剂，也是IL-1诱导胸腺细胞增殖的有效抑制剂。SV-IV与IL-1α形成非共价二聚体结合而不与IL-1β结合的能力、其诱导IL-1与胸腺细胞表面自身受体结合显著减少的能力以及其与巨噬细胞质膜特异性结合的能力，可能在这些抑制作用的分子机制中起重要作用。
