Tufano M A, Porta R, Farzati B, Di Pierro P, Rossano F, Catalanotti P, Baroni A, Metafora S
Institute of Microbiology, Medical School, 2nd University of Naples, Italy.
Cell Immunol. 1996 Mar 15;168(2):148-57. doi: 10.1006/cimm.1996.0061.
Micromolar amounts of SV-IV, one of the major proteins secreted from the rat seminal vesicle epithelium, induce in vitro a marked release of a variety of cytokines (interferon-gamma, tumor necrosis factor-alpha, interleukin 6, and granulocyte-monocyte colony-stimulating factor) from human resting peripheral blood mononuclear cells as well as from isolated resting lymphocytes and monocytes. This effect was found to be significantly higher when the spermidine adduct of SV-IV (Spd2-SV-IV), synthesized in vitro by the enzyme transglutaminase, was used instead of the native protein. Furthermore, the pretreatment of monocytes with transglutaminase caused an increase of the inducing effect of both native and modified SV-IV on the release of interleukin 6 from these cells. The inducing effect of these proteins on the cytokine release was markedly inhibited by actinomycin D and cycloheximide.
微摩尔量的精囊素 -IV（大鼠精囊上皮分泌的主要蛋白质之一）在体外可诱导人静息外周血单核细胞以及分离出的静息淋巴细胞和单核细胞显著释放多种细胞因子（γ-干扰素、肿瘤坏死因子-α、白细胞介素6和粒细胞 - 单核细胞集落刺激因子）。当使用通过转谷氨酰胺酶体外合成的精囊素 -IV的亚精胺加合物（Spd2 - SV - IV）代替天然蛋白质时，发现这种效应明显更高。此外，用转谷氨酰胺酶预处理单核细胞会导致天然和修饰的精囊素 -IV对这些细胞释放白细胞介素6的诱导作用增强。放线菌素D和环己酰亚胺可显著抑制这些蛋白质对细胞因子释放的诱导作用。
