Seeber S
Fortschr Med. 1977 Feb 3;95(5):295-8.
Leucocytes from normal donors and leukemia patients were isolated and lebelled in vitro with 32P-orthophosphate in order to compare labelling characteristics of nuclear high-molecular weight RNA, labelling characteristics, nucleotide compositions and oligonucleotide frequencies of ribosomal 28 S RNA. These studies revealed 1. structural microheterogeneity of 28 S RNA between the various leukemia cells studies without presenting a leukemia-specific structural marker, 2. an impaired production of ribosomal 28 S RNA from its nuclear precursor 45 S RNA in acute myeloblastic leukemia compared to PHA-stimulated normal lymphocytes. In the second part of this work, the influence of RNA from immunocompetent lymphocytes on the PHA-stimulation of M. Hodgkin lymphocytes was analyzed; the third part deals with studies on macromolecular carriers forcytostatic anthracyclines in human leukemia cells.
分离正常供体和白血病患者的白细胞，并在体外用32P-正磷酸盐进行标记，以比较核高分子量RNA的标记特性、核糖体28S RNA的标记特性、核苷酸组成和寡核苷酸频率。这些研究揭示了：1. 在各种白血病细胞研究中，28S RNA存在结构微异质性，但未呈现白血病特异性结构标记；2. 与PHA刺激的正常淋巴细胞相比，急性髓细胞白血病中核糖体28S RNA从其核前体45S RNA的产生受损。在这项工作的第二部分，分析了免疫活性淋巴细胞的RNA对霍奇金淋巴瘤淋巴细胞PHA刺激的影响；第三部分涉及人类白血病细胞中细胞毒性蒽环类药物大分子载体的研究。
