Rubin A D
J Clin Invest. 1971 Dec;50(12):2485-97. doi: 10.1172/JCI106749.
The kinetics of ribosomal RNA transcription and processing were assessed in chronic lymphocytic leukemia (CLL) lymphocytes during the initial phases of their delayed response to phytohemagglutinin. When compared to cultures of normal lymphocytes, CLL cultures developed normal augmentations in 45S rRNA precursor transcription and cleavage after a 1 hr incubation with PHA. However, failure to conserve 18S RNA subunits persisted in the CLL cultures. Subsequently the PHA-induced progressive rise in 45S RNA transcription became aborted and the over-all rate of RNA synthesis lagged far behind the levels attained by normal cultures incubated with PHA for 48 hr. CLL cultures responding to PHA in a delayed fashion exhibited efficient conservation of 18S RNA at 168 hr. In normal cultures, PHA-induced conservation of 18S RNA appeared to be independent of any effect on 45S ribosomal RNA precursor transcription. Therefore, the sluggish growth response of CLL lymphocytes was associated with a defect in one of the important mechanisms regulating assembly of new ribosomes.
在慢性淋巴细胞白血病（CLL）淋巴细胞对植物血凝素延迟反应的初始阶段，评估了核糖体RNA转录和加工的动力学。与正常淋巴细胞培养物相比，CLL培养物在与PHA孵育1小时后，45S rRNA前体转录和切割出现正常增强。然而，CLL培养物中18S RNA亚基的保存仍存在缺陷。随后，PHA诱导的45S RNA转录的逐渐增加被中止，RNA合成的总体速率远远落后于与PHA孵育48小时的正常培养物所达到的水平。以延迟方式对PHA作出反应的CLL培养物在168小时时表现出对18S RNA的有效保存。在正常培养物中，PHA诱导的18S RNA保存似乎与对45S核糖体RNA前体转录的任何影响无关。因此，CLL淋巴细胞生长反应迟缓与调节新核糖体组装的重要机制之一的缺陷有关。
