Krotkiewski H, Lisowska E, Nilsson G, Grönberg G, Nilsson B
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw.
Carbohydr Res. 1993 Feb 1;239:35-50. doi: 10.1016/0008-6215(93)84201-g.
Treatment of purified human glycophorin A with alkaline borohydride cleaved the oligosaccharide side chains to yield alditol derivatives that were separated by gel filtration into three mixtures of low molecular weight compounds. Each mixture was oxidised with periodate, and the products were reduced with borohydride and analysed after acetylation or methylation by GLC-MS and FABMS. The resulting data allowed the monosaccharide sequence and linkage positions to be assigned to each component of the mixtures. The anomeric configuration was determined by 1H NMR spectroscopy of the intact fractions. The structures of a desialylated tetrasaccharide, two monosialylated trisaccharides, and five other minor products were defined.
用碱性硼氢化物处理纯化的人血型糖蛋白A，可裂解寡糖侧链，生成糖醇衍生物，这些衍生物通过凝胶过滤分离成三种低分子量化合物混合物。每种混合物用过碘酸盐氧化，产物用硼氢化物还原，并在乙酰化或甲基化后通过气相色谱-质谱联用仪（GLC-MS）和快原子轰击质谱（FABMS）进行分析。所得数据可确定混合物各组分的单糖序列和连接位置。通过完整馏分的1H核磁共振光谱确定异头构型。确定了一种去唾液酸四糖、两种单唾液酸三糖和其他五种次要产物的结构。
