Mezquita J, López-Ibor B, Pau M, Mezquita C
Molecular Genetics Research Group, Faculty of Medicine, University of Barcelona, Spain.
FEBS Lett. 1993 Mar 22;319(3):244-8. doi: 10.1016/0014-5793(93)80555-9.
We have previously reported that the chicken polyubiquitin gene UbII is preferentially expressed during spermatogenesis and we show here that UbII is the predominant polyubiquitin gene expressed in early embryogenesis. Two main initiation sites were detected. Transcription from the initiation site used in early embryos results in the presence of an intron in the 5'-untranslated region of the transcripts as has been reported for other polyubiquitin messages. In mature testis, however, the use of a different initiation site, located within the intron, produces intronless transcripts. Distinct promoter sequences, present in each initiation site, may regulate the differential expression observed in this gene.
我们之前报道过，鸡多聚泛素基因UbII在精子发生过程中优先表达，并且我们在此表明UbII是早期胚胎发育中表达的主要多聚泛素基因。检测到两个主要的起始位点。正如其他多聚泛素信息所报道的那样，早期胚胎中使用的起始位点转录导致转录本的5'-非翻译区存在一个内含子。然而，在成熟睾丸中，位于内含子内的不同起始位点的使用产生了无内含子的转录本。每个起始位点中存在的不同启动子序列可能调节该基因中观察到的差异表达。
