通过X波段电子自旋共振检测吸入二叔丁基硝基苯后活体小鼠体内的自由基。

Detection of free radicals in living mice after inhalation of DTBN by X-band ESR.

作者信息

Mäder K, Stösser R, Borchert H H

机构信息

Department of Pharmacy, Humboldt University, Berlin, Germany.

出版信息

Free Radic Biol Med. 1993 Mar;14(3):339-42. doi: 10.1016/0891-5849(93)90030-x.

DOI:10.1016/0891-5849(93)90030-x

PMID:8384585

Abstract

A new application of X-band electron spin resonance (ESR) is demonstrated for in vivo investigations on living mice. It is the first report on in vivo detection, identification, and localization of a free radical after uptake in the body of a living mammal by inhalation. The volatile di-tert-butyl-nitroxyl (DTBN) has been used as a suitable spin probe. After inhalation of DTBN, a superimposed ESR spectrum consisting of a polar (g = 2.0057; a = 1.65 mT) and a nonpolar paramagnetic species (g = 2.0061; a = 1.51 mT) was detected in mice tails. Collected blood exhibits only the polar spectrum. The decay of signals after removing of the DTBN-soaked swab follows first-order kinetics with k = 0.08 min-1.

摘要

展示了X波段电子自旋共振（ESR）在对活体小鼠进行体内研究中的一种新应用。这是关于通过吸入在活体哺乳动物体内摄取自由基后进行体内检测、鉴定和定位的首次报告。挥发性二叔丁基硝基氧（DTBN）已被用作合适的自旋探针。吸入DTBN后，在小鼠尾巴中检测到由极性（g = 2.0057；a = 1.65 mT）和顺磁性非极性物质（g = 2.0061；a = 1.51 mT）组成的叠加ESR谱。采集的血液仅显示极性谱。去除浸泡有DTBN的棉签后信号的衰减遵循一级动力学，k = 0.08 min-1。