Kasperbauer J L, O'Halloran G L, Espy M J, Smith T F, Lewis J E
Department of Otolaryngology, Mayo Clinic, Rochester, Minn. 55905.
Laryngoscope. 1993 Apr;103(4 Pt 1):416-20. doi: 10.1002/lary.5541030409.
The incidence of human papillomavirus (HPV) DNA in archival formalin-fixed, paraffin-embedded tissue sections from verrucous carcinoma of the larynx was determined using polymerase chain reaction (PCR) with consensus primers and by in situ hybridization designed to detect HPV types 6/11, 16/18, 31/33/35. HPV DNA was detected in 17 (85%) of 20 tissue samples by PCR; none of the 20 samples were positive for the seven genotype types tested by in situ hybridization. PCR is a valuable tool to detect HPV and therefore will significantly clarify the importance of HPV in squamous mucosal disorders.
利用聚合酶链反应（PCR）和设计用于检测6/11型、16/18型、31/33/35型人乳头瘤病毒（HPV）的原位杂交技术，对喉疣状癌存档的福尔马林固定、石蜡包埋组织切片中的HPV DNA发生率进行了测定。通过PCR在20个组织样本中的17个（85%）检测到了HPV DNA；通过原位杂交检测的7种基因型中，20个样本均未呈阳性。PCR是检测HPV的一项重要工具，因此将显著阐明HPV在鳞状黏膜疾病中的重要性。
