Fliss D M, Noble-Topham S E, McLachlin M, Freeman J L, Noyek A M, van Nostrand A W, Hartwick R W
Department of Otolaryngology, Mount Sinai Hospital, Toronto, Ontario, Canada.
Laryngoscope. 1994 Feb;104(2):146-52. doi: 10.1288/00005537-199402000-00005.
Laryngeal verrucous carcinoma (LVC) is a rare, well-differentiated variant of squamous carcinoma with a low malignant potential. Human papillomavirus (HPV)-16 DNA has been identified in a small number of LVC and an etiologic relationship has been suggested. A correlative clinical and molecular pathological study was performed in order to determine the prevalence and typing of HPV DNA in LVC. Possible associations between patient and tumor subsets, and the presence of HPV DNA were also investigated. Formalin-fixed, paraffin-embedded tissue samples from 29 patients with LVC were examined by polymerase chain reaction (PCR) using DNA primers specific for HPV types 6b/11, 16, and 18. Overall, HPV DNA was detected in 13 (45%) of the cases. Of these, HPV-16 DNA, HPV-18 DNA, and both HPV-16 DNA and HPV-18 DNA were detected in 4 (14% overall; 31% of positive cases), 4, and 5 (17% overall; 38% of positive cases), respectively. HPV-6b/11 DNA was not detected in any LVCs. In 16 cases, no HPV DNA was detected. There was a trend toward HPV DNA detection in higher stage tumors. HPV DNA detection was unrelated to patient age, tumor site, or radiotherapeutic responsiveness. The detection of HPV DNA in 45% of LVCs suggests an association between the presence of HPV-16 DNA and HPV-18 DNA, and some LVCs.
喉疣状癌（LVC）是一种罕见的、高分化的鳞状细胞癌变体，恶性潜能较低。在少数喉疣状癌中已鉴定出人乳头瘤病毒（HPV）-16 DNA，并提示存在病因学关系。为了确定喉疣状癌中HPV DNA的患病率和类型，进行了一项相关的临床和分子病理学研究。还研究了患者和肿瘤亚组与HPV DNA存在之间的可能关联。使用针对HPV 6b/11、16和18型的DNA引物，通过聚合酶链反应（PCR）对29例喉疣状癌患者的福尔马林固定、石蜡包埋组织样本进行检测。总体而言，13例（45%）病例检测到HPV DNA。其中，分别有4例（总体14%；阳性病例的31%）检测到HPV-16 DNA、4例检测到HPV-18 DNA以及5例（总体17%；阳性病例的38%）同时检测到HPV-16 DNA和HPV-18 DNA。在任何喉疣状癌中均未检测到HPV-6b/11 DNA。16例病例未检测到HPV DNA。在更高分期的肿瘤中存在HPV DNA检测的趋势。HPV DNA检测与患者年龄、肿瘤部位或放射治疗反应性无关。45%的喉疣状癌中检测到HPV DNA提示HPV-16 DNA和HPV-18 DNA的存在与一些喉疣状癌之间存在关联。
