Brandsma J L, Steinberg B M, Abramson A L, Winkler B
Cancer Res. 1986 Apr;46(4 Pt 2):2185-8.
Verrucous carcinoma of the larynx clinically resembles laryngeal papilloma in that both are wart-like masses on the vocal cords and may be characterized by multifocality and recurrence. Human papillomavirus (HPV) infection is an etiological factor in laryngeal papilloma, and recent evidence implicates HPV in squamous neoplasias. To determine whether HPV is also associated with verrucous carcinoma of the larynx, we analyzed tissue specimens from six patients with verrucous carcinoma of the larynx by Southern and DNA dot blot hybridization for HPV DNA. From three patients, specimens of normal laryngeal epithelium were also studied. All tissues showed evidence of HPV sequences related but not identical to HPV-16. They were negative for HPV-11. In contrast, four squamous cell carcinomas of the larynx and three normal laryngeal tissues were negative for HPV DNA. Histological sections of the six verrucous lesions were found to contain koilocytosis. Immunoperoxidase staining for HPV capsid antigens was negative in all these cases. The consistent and specific association of HPV with the verrucous carcinomas in this report suggests the possibility of a pathogenic involvement.
喉疣状癌在临床上与喉乳头状瘤相似，因为两者在声带处均为疣状肿物，且都具有多灶性和复发性的特点。人乳头瘤病毒（HPV）感染是喉乳头状瘤的一个病因，最近有证据表明HPV与鳞状上皮肿瘤有关。为了确定HPV是否也与喉疣状癌相关，我们通过Southern杂交和DNA斑点杂交对6例喉疣状癌患者的组织标本进行HPV DNA分析。另外，还对3例患者的正常喉上皮标本进行了研究。所有组织均显示出与HPV-16相关但并不相同的HPV序列证据。这些组织HPV-11检测呈阴性。相比之下，4例喉鳞状细胞癌和3例正常喉组织的HPV DNA检测均为阴性。6例疣状病变的组织学切片均发现有挖空细胞。所有这些病例的HPV衣壳抗原免疫过氧化物酶染色均为阴性。本报告中HPV与疣状癌的一致且特异性关联提示了其致病作用的可能性。
