Voytek P, Kozak C A
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Virology. 1989 Nov;173(1):58-67. doi: 10.1016/0042-6822(89)90221-3.
HoMuLV is an NB-tropic wild mouse leukemia virus (MuLV) with ecotropic host range which induces lymphoma, erythroleukemia, and myelogenous leukemia in NIH Swiss mice. Although HoMuLV uses the same cell surface receptor as other ecotropic MuLVs, hybridization studies suggested that the HoMuLV envelope glycoprotein differs from that of other ecotropic MuLVs. We have now molecularly cloned HoMuLV and sequenced its LTR, gag, and env regions. HoMuLV differs markedly from other MuLVs in the LTR U3 region, in the SU protein of env, and in p12gag. U3 contains a single copy of a sequence analogous to the direct repeat found in other LTRs, and this region includes several previously defined protein binding sites. The predicted amino acid sequence for the coding regions of env and gag reveal that p12 and the SU protein show less than 59 and 65% sequence identity, respectively, with those of other MuLVs. A 0.6-kb segment of the 5' region of the HoMuLV env was used as a hybridization probe to examine inbred and wild mouse genomic DNAs for proviral sequences. HoMuLV env sequences were not present in the germline of any of the inbred strains or wild mice examined including the Mus hortulanus mice which harbored infectious virus. Therefore, HoMuLV represents an evolutionarily related, but distinct, subgroup of ecotropic MuLV which is not genetically transmitted in its natural host.
霍姆氏白血病病毒（HoMuLV）是一种嗜NZB小鼠的野生型小鼠白血病病毒（MuLV），具有嗜亲性宿主范围，可在NIH瑞士小鼠中诱发淋巴瘤、红白血病和髓性白血病。尽管HoMuLV与其他嗜亲性MuLV使用相同的细胞表面受体，但杂交研究表明，HoMuLV包膜糖蛋白与其他嗜亲性MuLV的不同。我们现已对HoMuLV进行了分子克隆，并对其长末端重复序列（LTR）、gag和env区域进行了测序。HoMuLV在LTR U3区域、env的表面糖蛋白（SU）和p12gag方面与其他MuLV有显著差异。U3包含一个与其他LTR中发现的直接重复序列类似的序列单拷贝，该区域包括几个先前确定的蛋白质结合位点。env和gag编码区域的预测氨基酸序列显示，p12和SU蛋白与其他MuLV的序列同一性分别低于59%和65%。HoMuLV env 5'区域的一个0.6 kb片段用作杂交探针，以检测近交系和野生小鼠基因组DNA中的前病毒序列。在所检测的任何近交系或野生小鼠（包括携带传染性病毒的短尾小鼠）的种系中均未发现HoMuLV env序列。因此，HoMuLV代表了嗜亲性MuLV的一个进化相关但不同的亚组，它在其自然宿主中不会进行基因传递。
