Mathias S L, Scott A F
Center for Medical Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21287-4925.
Biochem Biophys Res Commun. 1993 Mar 15;191(2):625-32. doi: 10.1006/bbrc.1993.1263.
Human Long Interspersed elements (LINEs or L1Hs elements) are a highly repetitive class of DNA sequences which are dispersed by retrotransposition. Full length L1Hs transcripts and L1-encoded proteins have been shown by others to be present in NTera2D1 and other related teratocarcinoma cell lines. Using standard methods for the identification of DNA binding proteins we identified two proteins or complexes of proteins which specifically bind to the L1Hs promoter region and are qualitatively different between NTera2D1 and HeLa cell nuclear extracts. These proteins represent candidates for the factors controlling the differential expression of L1 sequences.
人类长散在重复序列（LINEs或L1Hs元件）是一类高度重复的DNA序列，通过逆转座作用散布。其他人已证明全长L1Hs转录本和L1编码的蛋白质存在于NTera2D1和其他相关的畸胎瘤细胞系中。我们使用鉴定DNA结合蛋白的标准方法，鉴定出两种蛋白质或蛋白质复合物，它们特异性结合L1Hs启动子区域，并且在NTera2D1和HeLa细胞核提取物之间存在质的差异。这些蛋白质代表了控制L1序列差异表达的因子的候选物。
